characterization of aflatoxigenic aspergillus flavus and a. parasiticus strain isolates from animal feedstuffs in northeastern iran

نویسندگان

e. davari

m. mohsenzadeh

gh. mohammadi

r. rezaeian-doloei

چکیده

aflatoxins are secondary toxic metabolites produced by some aspergillus spp. particularly, aspergillus flavus and a. parasiticus that contaminate food and feed. the objective of this study was to evaluate the contamination of feedstuffs with aspergillus spp. and detect genes involved in the aflatoxin biosynthesis pathway of a. flavus and a. parasiticus isolates. a total of 110 cow feed samples (comprised of silage, concentrate, hay and total mixed ration) from 30 industrial and semi-industrial dairy farms of khorasan razavi province, northeastern iran, were examined using cultural and pcr methods. 68 (61.82%) aspergillus spp. were isolated from 110 samples of feedstuff. the predominant aspergillus isolates were a. fumigates (21.81%), followed by a. flavus (17.27%), a. niger (10%), a. parasiticus (8.18%), and a. oryzae (4.54%). fungal contamination levels of industrial and semi-industrial dairy farm samples were not significantly different (p>0.05). using four sets of primers, a quadruplex pcr was developed to detect genes (nor1, ver1, omta and aflr) at different loci coding enzymes in the aflatoxin biosynthesis pathway of a. flavus and a. parasiticus strains. out of 28 strains of a. flavus and a. parasiticus, 10 isolates (35.71%) showed a quadruplet pattern indicating the important genes involved in the aflatoxin biosynthesis pathway, encoded for functional products. these isolates were confirmed to be aflatoxigenic by thin layer chromatography. 18 isolates (64.29%) had three, two and single molecular patterns. the results obtained by this study show that rapid and specific detection of aflatoxigenic molds is important to ensure the microbiological safety of feedstuffs.

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منابع مشابه

Characterization of aflatoxigenic Aspergillus flavus and A. parasiticus strain isolates from animal feedstuffs in northeastern Iran

Aflatoxins are secondary toxic metabolites produced by some Aspergillus spp. particularly, Aspergillus flavus and A. parasiticus that contaminate food and feed. The objective of this study was to evaluate the contamination of feedstuffs with Aspergillus spp. and detect genes involved in the aflatoxin biosynthesis pathway of A. flavus and A. parasiticus isolates. A total of 110 cow feed samples ...

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Characterization of aflatoxigenic Aspergillus flavus and A. parasiticus strain isolates from animal feedstuffs in northeastern Iran.

Aflatoxins are secondary toxic metabolites produced by some Aspergillus spp. particularly, Aspergillus flavus and A. parasiticus that contaminate food and feed. The objective of this study was to evaluate the contamination of feedstuffs with Aspergillus spp. and detect genes involved in the aflatoxin biosynthesis pathway of A. flavus and A. parasiticus isolates. A total of 110 cow feed samples ...

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Background and Purpose: Aflatoxins are naturally produced by some species of Aspergillus, such as A. flavus and A. parasiticus. Aflatoxins reportedly have carcinogenic effects on human, poultry, and livestock, and therefore could be linked to severe human illnesses. Aflatoxin biosynthesis pathway involves different clustered genes, including structural, regular, and unassigned genes. The presen...

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عنوان ژورنال:
iranian journal of veterinary research

ناشر: shiraz university

ISSN 1728-1997

دوره 16

شماره 2 2015

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